Long-term ph: ph interval where the medium can be operated without significant change of function. (BSA) and for Capto Q ImpRes: 5 mm sodium acetate, ph 4.75 (BSA and β-lactoglobulin) Short-term ph: ph interval where the medium can be subjected to cleaning or sanitization-in-place without significant change of function. Buffer conditions used were for Capto SP ImpRes: 2 mm sodium phosphate, ph 7.2 (lysozyme) and 5 mm Tris, ph 8. Dynamic binding capacity at 1% breakthrough measured at a residence time of 4 min (15 cm/h) in a Tricorn 5/1 column with 1 cm bed height. Flow velocity stated in the Table is dependent on the column used, see Table 3. Main characteristics of Capto SP ImpRes and Capto Q ImpRes Capto SP ImpRes Capto Q ImpRes Matrix High-flow agarose High-flow agarose Functional group - CH 2 CH 2 CH 2 SO 3 CH 2 N + ( CH 3 ) 3 Total ionic capacity.13 to.16 mmol (H + )/ml medium.15 to.18 mmol (Cl - )/ml medium Average particle size (d 5v )* 4 μm 4 μm Flow velocity At least 22 cm/h in a 1 m diameter column with bed height 2 cm at 2 C measured using process buffers with the same viscosity as water at 7 mg lysozyme/ml medium, > 95 mg BSA/mL medium > 55 mg BSA/mL medium, > 48 mg β-lactoglobulin/ml medium Operating ph stability short term 3 to 14 2 to 14 long term 4 to 12 2 to 12 Working temperature 4 C to 3 C 4 C to 3 C Chemical stability All commonly used aqueous buffers, 1 M sodium hydroxide, 8 M urea, 6 M guanidine hydrochloride, 3% isopropanol, and 7% ethanol Avoid Oxidizing agents, cationic detergents Oxidizing agents, anionic detergents Storage 2% ethanol.2 M sodium acetate at 4 C to 3 C 2% ethanol at 4 C to 3 C * d 5v is the average particle size of the cumulative volume distribution. The strong ion exchange ligand groups of Capto SP ImpRes (left) and Capto Q ImpRes (right) are well established in large-scale purifications. Table 1 lists further characteristics of the media.
These features, in combination with the well-established SP and Q ligands, make Capto SP ImpRes and Capto Q ImpRes an excellent choice for reliable intermediate purification and polishing in a commercial setting. Capto ImpRes media are based on a high-flow agarose base matrix with good pressure/flow properties (Fig 3) and a small bead size (approx. Capto SP ImpRes and Capto Q ImpRes extend the well-established Capto platform to include high-resolution media for late intermediate purification and polishing.
The ligand of Capto SP ImpRes is a sulfonate group while the ligand of Capto Q ImpRes is a quaternary amine group (Fig 2).
Key benefits of Capto SP ImpRes and Capto Q ImpRes include: High-resolution intermediate purification and polishing based on the well-established Capto platform with traditional ligands Flexible process design due to large operational window of flow rates and bed heights High-throughput purifications easy to optimize and scale up Higher manufacturing productivity enables improved process economy Security of supply and comprehensive regulatory support Chromatography media characteristics Ionic groups and base matrix Capto SP ImpRes and Capto Q ImpRes are strong cation and strong anion exchangers, respectively. The ability to run at higher flow rates and higher bed heights increases productivity and flexibility in process design. By combining the high-flow characteristics of Capto media with a small particle size, Capto SP ImpRes and Capto Q ImpRes deliver excellent pressure-flow properties with excellent resolution. Both chromatography media (resins) are part of platform of high-resolution media based on the Capto product line. 1 GE Healthcare Life Sciences Data file AC Ion exchange chromatography Capto SP ImpRes Capto Q ImpRes Capto SP ImpRes and Capto Q ImpRes are strong cation and strong anion exchangers for the high-throughput intermediate purification and polishing steps of a wide range of biomolecules.
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